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Sperm DNA Fragmentation Test:
This test can be used to find out whether decrease in fertilisation potential has relation to lack of DNA.
Procedure: Take 100 micro litre liquefied semen in a test tube. add 1 ml wash solution, mix well and centrifuge at 1500 RPM for 5 minutes. discard all the supernatant without disturbing the pellet. properly dissolve NCD powder in 10 ml distilled water. add 200 micro litre of this NCD solution to the pellet and mix well. keep at room temperature for 30 minutes. add 50 - 100 micro litre stop solution and transfer a drop of mixture solution on the glass slide and covered with a cover slip. examine under 40x objective and count condensed and decondensed head.
Normal range of decondensed head should be more than 70%.